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pbs  (Native Antigen Inc)


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    Native Antigen Inc pbs
    Pbs, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Native Antigen Inc
    Average 92 stars, based on 10 article reviews
    pbs - by Bioz Stars, 2026-03
    92/100 stars

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    Detection of recombinant dengue NS1 antigen using Rabbit Polyclonal Antibodies against the Dengue NS1 antigen (RPAD). The ELISA plate was coated with rDNS1Ag (100 ng/well). To this, polyclonal antibodies raised in rabbits were added at different dilutions (1:50 to 1:50000 dilutions). The antibody response was detected by the secondary antibody, anti-rabbit-HRP (1:50000; 50 µl/well). Absorbance (OD) was measured at 452 nm. ns - statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

    Journal: Scientific Reports

    Article Title: Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen

    doi: 10.1038/s41598-026-35952-1

    Figure Lengend Snippet: Detection of recombinant dengue NS1 antigen using Rabbit Polyclonal Antibodies against the Dengue NS1 antigen (RPAD). The ELISA plate was coated with rDNS1Ag (100 ng/well). To this, polyclonal antibodies raised in rabbits were added at different dilutions (1:50 to 1:50000 dilutions). The antibody response was detected by the secondary antibody, anti-rabbit-HRP (1:50000; 50 µl/well). Absorbance (OD) was measured at 452 nm. ns - statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

    Article Snippet: For developing the RPAD-ELISA, ELISA plates (Costar, Corning, USA) were coated with different concentrations of recombinant dengue NS1 antigens (DENV-1 to 4, Japanese encephalitis virus [JEV], tick-borne encephalitis virus [TBEV], West-Nile virus [WNV] and yellow fever virus [YFV]; The Native Antigen Company, Oxfordshire, UK: SKU: REC31778 –1781).

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Concentration Assay

    The RPAD is sensitive to detect all the four serotypes of dengue virus. The ELISA plate was coated with rDNS1Ag (100 ng/well) of different serotypes of DENV (1–4). To this, polyclonal antibody, RPAD (1:5000) was added. The binding of polyclonal antibodies was detected by anti-rabbit-HRP. Absorbance (OD) was measured at 452 nm in an ELISA reader. D1 to D4 –NS1 antigen of Dengue virus serotypes, D2 (R&D) – NS1 antigen used for immunizing the rabbits. ns- statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

    Journal: Scientific Reports

    Article Title: Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen

    doi: 10.1038/s41598-026-35952-1

    Figure Lengend Snippet: The RPAD is sensitive to detect all the four serotypes of dengue virus. The ELISA plate was coated with rDNS1Ag (100 ng/well) of different serotypes of DENV (1–4). To this, polyclonal antibody, RPAD (1:5000) was added. The binding of polyclonal antibodies was detected by anti-rabbit-HRP. Absorbance (OD) was measured at 452 nm in an ELISA reader. D1 to D4 –NS1 antigen of Dengue virus serotypes, D2 (R&D) – NS1 antigen used for immunizing the rabbits. ns- statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

    Article Snippet: For developing the RPAD-ELISA, ELISA plates (Costar, Corning, USA) were coated with different concentrations of recombinant dengue NS1 antigens (DENV-1 to 4, Japanese encephalitis virus [JEV], tick-borne encephalitis virus [TBEV], West-Nile virus [WNV] and yellow fever virus [YFV]; The Native Antigen Company, Oxfordshire, UK: SKU: REC31778 –1781).

    Techniques: Virus, Enzyme-linked Immunosorbent Assay, Binding Assay, Concentration Assay

    Evaluation of cross-reactivity of RPAD with the NS1 antigen of other Flaviviruses. The ELISA plate was coated with NS1 Ag of flavivirus (100 ng/well). To this, polyclonal antibody, RPAD (1:5000) was added. The binding of RPAD was detected by anti-rabbit-HRP. JE-Japanese Encephalitis Virus; TBEV Tick-borne Encephalitis Virus; WNV-West-Nile Virus; YFV-Yellow Fever Virus, D2 (R&D) – NS1 antigen used for immunizing the rabbits. ns- statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

    Journal: Scientific Reports

    Article Title: Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen

    doi: 10.1038/s41598-026-35952-1

    Figure Lengend Snippet: Evaluation of cross-reactivity of RPAD with the NS1 antigen of other Flaviviruses. The ELISA plate was coated with NS1 Ag of flavivirus (100 ng/well). To this, polyclonal antibody, RPAD (1:5000) was added. The binding of RPAD was detected by anti-rabbit-HRP. JE-Japanese Encephalitis Virus; TBEV Tick-borne Encephalitis Virus; WNV-West-Nile Virus; YFV-Yellow Fever Virus, D2 (R&D) – NS1 antigen used for immunizing the rabbits. ns- statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

    Article Snippet: For developing the RPAD-ELISA, ELISA plates (Costar, Corning, USA) were coated with different concentrations of recombinant dengue NS1 antigens (DENV-1 to 4, Japanese encephalitis virus [JEV], tick-borne encephalitis virus [TBEV], West-Nile virus [WNV] and yellow fever virus [YFV]; The Native Antigen Company, Oxfordshire, UK: SKU: REC31778 –1781).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Virus, Concentration Assay